Differential patterning of cells in adult mouse brain mapped with ISH identified by Gfap+Agt+Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Unc13c+Agt-Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Gfap-Agt+Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Unc13c-Agt+Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Frzb+Ascl1+Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Frzb+(Nes+)Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Ogt-Fam107a+Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.

Differential patterning of cells in adult mouse brain mapped with ISH identified by Mfge8+Slc1a3+ marker combination in adult mouse brain. Mapping is done based on multiplex ISH preformed on brain sections from animals of age P56-P60. Top left is an example of a brain section with the mirrored reference atlas. Black dots on the section show putative location of a subtype identified by co-localization of corresponding biomarker (scale bar, 1000μm). In the boxes below markers used to define a subtype are shown separately (on the top of the DAPI signal in blue) followed by a merged image (scale bar, 10μm). Barplots on the right show in situ single cell RNA marker counts per astrocytes (Slc1a3+ cells) followed by the dis-tribution of the subtype among brain regions and the normaized distribution of the subtype by the number of astrocytes (Glast encoding Slc1a3+ cells) per brain region. In the barplots astrocytes belonging to the subtype of interest are highlighted with the corresponding color, counts per marker are sorted in the descending order starting from the right. Error bars represent SEM averaged across all stained sections.